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fusion fx edge  (VILBER GmbH)


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    Structured Review

    VILBER GmbH fusion fx edge
    Fusion Fx Edge, supplied by VILBER GmbH, used in various techniques. Bioz Stars score: 99/100, based on 13121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fusion+fx7/FUSION+FX+EDGE/custom%40fusion-fx-edge-1%4042860544
    Average 99 stars, based on 13121 article reviews
    fusion fx edge - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Neprilysin inhibition reduces microtubule detyrosination in cardiomyocytes through a cGMP-PRKG1-VASH1 axis
    Article Snippet: .. Secondary antibody ( Table S2 ) signals were captured with ECL solution or appropriate fluorescence excitation and detection on the FUSION FX7 (Vilber). .. The fluorescently labelled GAPDH680 (Invitrogen, MA5-15738-D680) loading control antibody was incubated for 1 h at room temperature.

    Software:

    Article Title: A fully iPS-cell-derived 3D model of the human blood-brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions.
    Article Snippet: Statistical significance was analyzed using GraphPad Prism, except for proteomics analysis, where Perseus (v1.6.2.3) and Excel (v16.9) were used. .. Software and code Policy information about availability of computer code Data collection Zeiss Confocal microscope (LSM880 and LSM980) using 10x, 40x and 63x objectives; laser speckle contrast imager (LSCI, Perimed, Järfälla, Sweden); two-photon microscope (7MP, Carl Zeiss AG, Germany); LaVision UltraII microscope coupled to a white light laser module (NKT SuperK Extreme EXW-12); Fusion FX7 (Vilber Lourmat); Crossbeam Gemini 340 SEM (Zeiss); Illumina HiSeq4000; 3T nanoScan PET/MR 3T scanner equipped with a surface coil optimized for the mouse head (Mediso, Hungary); nanoElute nanoHPLC which was coupled to a TimsTOF pro mass spectrometer with a CaptiveSpray ion source (Bruker, Germany); BD FACSAriaIII. .. Data analysis Maxquant (version 1.6.17.), DIA-NN (Version 1.8), and DAVID (v2023q4) analysis of proteomic data and statistics; ImageJ (version 1.52p) for image and gel analysis; Fiji TrakEM2 (Ref 91) for serial EM section analysis; FACSDiva version 8.0.1 for flow cytometry; VesSAP pipeline (Ref: 45); Python (version 3.9) and R (version 4.0.0) for single-cell data analysis; NGS pipeline (https://github.com/GunnarSchotta/NGS.analysis) for ChIP-seq analysis; MATLAB (R2016b and R2020a) for blood flow data analysis, GraphPad (8.3.1), Excel (2016), and Adobe Illustrator (2017) for statistics and figure representation.

    Article Title: Cysteine Attenuates Intestinal Inflammation by Regulating the Gut Microbiota and TLR4-JNK/MAPK-NF-κB Pathway in Piglets
    Article Snippet: Briefly, equal amounts of protein (30 μg) were loaded onto sodium dodecyl sulfate polyacrylamide gels and then blotted onto polyvinylidene difluoride membranes (IPVH00010, 0.45 μm, Merck Co., Inc., Hunterton, NJ, USA). .. The membranes were incubated with primary antibodies (1:1000 dilution) at 4 °C overnight and then with an HRP-conjugated secondary antibody (1:5000 dilution) for 1 h at 25 °C, followed by detection with Fusion FX7 (VILBER, Collégien, France) and quantification using Fusion software (16.0.9.0). ..

    Microscopy:

    Article Title: A fully iPS-cell-derived 3D model of the human blood-brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions.
    Article Snippet: Statistical significance was analyzed using GraphPad Prism, except for proteomics analysis, where Perseus (v1.6.2.3) and Excel (v16.9) were used. .. Software and code Policy information about availability of computer code Data collection Zeiss Confocal microscope (LSM880 and LSM980) using 10x, 40x and 63x objectives; laser speckle contrast imager (LSCI, Perimed, Järfälla, Sweden); two-photon microscope (7MP, Carl Zeiss AG, Germany); LaVision UltraII microscope coupled to a white light laser module (NKT SuperK Extreme EXW-12); Fusion FX7 (Vilber Lourmat); Crossbeam Gemini 340 SEM (Zeiss); Illumina HiSeq4000; 3T nanoScan PET/MR 3T scanner equipped with a surface coil optimized for the mouse head (Mediso, Hungary); nanoElute nanoHPLC which was coupled to a TimsTOF pro mass spectrometer with a CaptiveSpray ion source (Bruker, Germany); BD FACSAriaIII. .. Data analysis Maxquant (version 1.6.17.), DIA-NN (Version 1.8), and DAVID (v2023q4) analysis of proteomic data and statistics; ImageJ (version 1.52p) for image and gel analysis; Fiji TrakEM2 (Ref 91) for serial EM section analysis; FACSDiva version 8.0.1 for flow cytometry; VesSAP pipeline (Ref: 45); Python (version 3.9) and R (version 4.0.0) for single-cell data analysis; NGS pipeline (https://github.com/GunnarSchotta/NGS.analysis) for ChIP-seq analysis; MATLAB (R2016b and R2020a) for blood flow data analysis, GraphPad (8.3.1), Excel (2016), and Adobe Illustrator (2017) for statistics and figure representation.

    Positron Emission Tomography:

    Article Title: A fully iPS-cell-derived 3D model of the human blood-brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions.
    Article Snippet: Statistical significance was analyzed using GraphPad Prism, except for proteomics analysis, where Perseus (v1.6.2.3) and Excel (v16.9) were used. .. Software and code Policy information about availability of computer code Data collection Zeiss Confocal microscope (LSM880 and LSM980) using 10x, 40x and 63x objectives; laser speckle contrast imager (LSCI, Perimed, Järfälla, Sweden); two-photon microscope (7MP, Carl Zeiss AG, Germany); LaVision UltraII microscope coupled to a white light laser module (NKT SuperK Extreme EXW-12); Fusion FX7 (Vilber Lourmat); Crossbeam Gemini 340 SEM (Zeiss); Illumina HiSeq4000; 3T nanoScan PET/MR 3T scanner equipped with a surface coil optimized for the mouse head (Mediso, Hungary); nanoElute nanoHPLC which was coupled to a TimsTOF pro mass spectrometer with a CaptiveSpray ion source (Bruker, Germany); BD FACSAriaIII. .. Data analysis Maxquant (version 1.6.17.), DIA-NN (Version 1.8), and DAVID (v2023q4) analysis of proteomic data and statistics; ImageJ (version 1.52p) for image and gel analysis; Fiji TrakEM2 (Ref 91) for serial EM section analysis; FACSDiva version 8.0.1 for flow cytometry; VesSAP pipeline (Ref: 45); Python (version 3.9) and R (version 4.0.0) for single-cell data analysis; NGS pipeline (https://github.com/GunnarSchotta/NGS.analysis) for ChIP-seq analysis; MATLAB (R2016b and R2020a) for blood flow data analysis, GraphPad (8.3.1), Excel (2016), and Adobe Illustrator (2017) for statistics and figure representation.

    Mass Spectrometry:

    Article Title: A fully iPS-cell-derived 3D model of the human blood-brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions.
    Article Snippet: Statistical significance was analyzed using GraphPad Prism, except for proteomics analysis, where Perseus (v1.6.2.3) and Excel (v16.9) were used. .. Software and code Policy information about availability of computer code Data collection Zeiss Confocal microscope (LSM880 and LSM980) using 10x, 40x and 63x objectives; laser speckle contrast imager (LSCI, Perimed, Järfälla, Sweden); two-photon microscope (7MP, Carl Zeiss AG, Germany); LaVision UltraII microscope coupled to a white light laser module (NKT SuperK Extreme EXW-12); Fusion FX7 (Vilber Lourmat); Crossbeam Gemini 340 SEM (Zeiss); Illumina HiSeq4000; 3T nanoScan PET/MR 3T scanner equipped with a surface coil optimized for the mouse head (Mediso, Hungary); nanoElute nanoHPLC which was coupled to a TimsTOF pro mass spectrometer with a CaptiveSpray ion source (Bruker, Germany); BD FACSAriaIII. .. Data analysis Maxquant (version 1.6.17.), DIA-NN (Version 1.8), and DAVID (v2023q4) analysis of proteomic data and statistics; ImageJ (version 1.52p) for image and gel analysis; Fiji TrakEM2 (Ref 91) for serial EM section analysis; FACSDiva version 8.0.1 for flow cytometry; VesSAP pipeline (Ref: 45); Python (version 3.9) and R (version 4.0.0) for single-cell data analysis; NGS pipeline (https://github.com/GunnarSchotta/NGS.analysis) for ChIP-seq analysis; MATLAB (R2016b and R2020a) for blood flow data analysis, GraphPad (8.3.1), Excel (2016), and Adobe Illustrator (2017) for statistics and figure representation.

    other:

    Article Title: A fully iPS-cell-derived 3D model of the human blood-brain barrier for exploring neurovascular disease mechanisms and therapeutic interventions.
    Article Snippet: Cells were treated with 10-μM DAF-2DA diluted in phenol-free medium for 24 h. After incubation, cells were fixed with 4% PFA and mounted with Fluoromount medium (Sigma-Aldrich, F4680-25ML) for imaging.

    Article Title: The stroke risk gene Foxf2 maintains brain endothelial cell function via Tie2 signaling
    Article Snippet: Cells were treated with 10-μM DAF-2DA diluted in phenol-free medium for 24 h. After incubation, cells were fixed with 4% PFA and mounted with Fluoromount medium (Sigma-Aldrich, F4680-25ML) for imaging.

    Incubation:

    Article Title: Cysteine Attenuates Intestinal Inflammation by Regulating the Gut Microbiota and TLR4-JNK/MAPK-NF-κB Pathway in Piglets
    Article Snippet: Briefly, equal amounts of protein (30 μg) were loaded onto sodium dodecyl sulfate polyacrylamide gels and then blotted onto polyvinylidene difluoride membranes (IPVH00010, 0.45 μm, Merck Co., Inc., Hunterton, NJ, USA). .. The membranes were incubated with primary antibodies (1:1000 dilution) at 4 °C overnight and then with an HRP-conjugated secondary antibody (1:5000 dilution) for 1 h at 25 °C, followed by detection with Fusion FX7 (VILBER, Collégien, France) and quantification using Fusion software (16.0.9.0). ..

    Article Title: ER-stress signaling and Alzheimer’s proteins adjust the quality of human protein synthesis
    Article Snippet: .. After washing and secondary antibody incubation, the Western blot was developed using the SuperSignalTM Western Blot Substrate (Thermo Fisher Scientific, A43840) with the Fusion Fx7 (Vilber) imaging system. ..

    Western Blot:

    Article Title: NCKAP1 coordinates focal adhesion and adherens junction dynamics in endothelial barrier maintenance and angiogenesis
    Article Snippet: .. 732 Western Bright Quantum ECL HRP substrate was added onto membranes, and signals were obtained 733 using a Fusion-FX7 (Vilber Lourmat, Eberhardzell, Germany). .. 734 Quantitative real-time PCR 735 At 48 hours post-transfection, cells were harvested and lysed for RNA isolation using Macherey-Nagel kits 736 according to the manufacturer's protocol.

    Article Title: The unfolded protein response and its activation by insulin in muscle are not altered by obesity or type 2 diabetes
    Article Snippet: The 221 secondary HRP-conjugated antibodies used were: Anti-rabbit (1:5000, #P0448, DakoCytomation, Denmark) 222 and anti-mouse (1:5000, #P0161, DakoCytomation, Denmark). .. 223 Protein bands in the discovery study cohort were visualized using Fusion FX7 (Vilber Lourmat, 224 Germany) after probing with enhanced chemiluminescence (Luminate Forte Western HRP Substrate, Millipore 225 #WBLUF0500, MA or SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific #34096, 226 D o w n l o a d e d f r o m h t t p : / / p o r t l a n d p r e s s . c o m / c l i n s c i / a r t i c l e - p d f / d o i / 1 0 . ..

    Article Title: ER-stress signaling and Alzheimer’s proteins adjust the quality of human protein synthesis
    Article Snippet: .. After washing and secondary antibody incubation, the Western blot was developed using the SuperSignalTM Western Blot Substrate (Thermo Fisher Scientific, A43840) with the Fusion Fx7 (Vilber) imaging system. ..

    Imaging:

    Article Title: ER-stress signaling and Alzheimer’s proteins adjust the quality of human protein synthesis
    Article Snippet: .. After washing and secondary antibody incubation, the Western blot was developed using the SuperSignalTM Western Blot Substrate (Thermo Fisher Scientific, A43840) with the Fusion Fx7 (Vilber) imaging system. ..



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